The response of colon cancer to chemotherapy was assessed relating to Response Evaluation Requirements in Solid Tumors criteria 7 ; the response of rectal malignancy to chemoradiation was assessed on the basis of histologic characteristics, as previously described.8 Written informed consent was obtained from all patients before enrollment in the scientific trial . Cell Culture The colon-cancer cell lines LoVo and DLD-1 were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen; the relative lines SW480, HT-29, HCT-116, and Caco-2 were acquired from the American Type Tradition Collection. Cells were cultured in standard medium and treated with aza-2-deoxycytidine or chemotherapeutic agents as explained in the Supplementary Appendix.This is because with the intensive training the muscle tissue are taken to exhaustion or overload point more speedily. As you train and go into exhaustion you will recognize that the muscles are unable to continue after a particular time and weight limit. This is the point of fatigue and it means that the muscles will be ready to recuperate and grow. Technique number 2 2 has all to do with eating a natural diet that’s rich in proteins. The body’s muscles are constructed with protein and therefore they can only be built through protein.